Italy earned the bronze medal.
Australia took the gold and set a new Olympic record with 3:28.92, and China earned bronze.
She is the most decorated of any female swimmer in history, having earned six gold medals.
Gold Medalists Jack Alexy, Chris Guiliano, Hunter Armstrong and Caeleb Dressel of Team United States stand for the national anthem during the Medal Ceremony on day one of the Olympic Games Paris 2024.Maddie Meyer/Getty Images
Her bronze win on Saturday makes it her 11th overall Olympic medal.
Ledecky will also compete in the 800 and 1,500 races, where she is favored to take gold.